基底膜

2020.06.26 Friday 19:56
0

    Duke大学のDavidの新作。

     

    これはすごいわ。。さすがDave。彼はいつも美しい論文をだす人だ。

    このプロジェクトを参考にして、なんとか独自路線を切り開こうと思います。

     

     

    Comprehensive Endogenous Tagging of Basement Membrane Components Reveals Dynamic Movement Within the Matrix Scaffolding

     

    Abstract

    Basement membranes (BMs) are supramolecular matrices built on laminin and type IV collagen networks that provide structural and signaling support to tissues. BM complexity, however, has hindered an understanding of its formation, dynamics, and regulation. Using genome editing, we tagged 29 BM matrix components and receptors in C. elegans with mNeonGreen. Here, we report a common template that initiates BM formation, which rapidly diversifies during tissue differentiation. Through photobleaching studies, we show that BMs are not static-surprisingly, many matrix proteins move within the laminin and collagen scaffoldings. Finally, quantitative imaging, conditional knockdown, and optical highlighting indicate that papilin, a poorly studied glycoprotein, is the most abundant component in the gonadal BM, where it facilitates type IV collagen removal during BM expansion and tissue growth. Together, this work introduces methods for holistic investigation of BM regulation and reveals that BMs are highly dynamic and capable of rapid change to support tissues.

     

    category:実験 | by:shinjicomments(0) | -
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