なに、これ、すごく面白そうな論文!

2018.02.15 Thursday 21:45
0

    Chishholmのとこからの論文、すごく面白そうな内容だ。

    Genetics誌だから、遺伝的相互作用を見ただけだと思うけど、これは研究の夢が広がるなあ。

    構造物である基底膜タンパク質の欠損でもgentic suppressorが取れることにびっくりです。。

    同時に、自分のアプローチもそれほど間違っていないのかな、と思える論文です。こういった遺伝的相互作用をきちんとみる地道な論文は私は好きです。

     

    vab-10let-805かあ、懐かしい遺伝子名だ。

     

    はやく、採点を終わらせてじっくり読もう。

     

    Genetic Suppression of Basement Membrane Defects in Caenorhabditis elegans by Gain of Function in Extracellular Matrix and Cell-Matrix Attachment Genes.

     

    Basement membranes are extracellular matrices essential for embryonic development in animals. Peroxidasins are extracellular peroxidases implicated in the unique sulfilimine crosslinks between type IV basement membrane collagens. Loss of function in the C. elegans peroxidasin PXN-2 results in fully penetrant embryonic or larval lethality. Using genetic suppressor screening we find that the requirement for PXN-2 in development can be bypassed by gain of function in multiple genes encoding other basement membrane components, or proteins implicated in cell-matrix attachment. We identify multiple alleles of let-805, encoding the transmembrane protein Myotactin, which suppress phenotypes of pxn-2 null mutants and of other basement membrane mutants such as F-spondin/spon-1 These let-805 suppressor alleles cause missense alterations in two pairs of FNIII repeats in the extracellular domain; they act dominantly and have no detectable phenotypes alone, suggesting they cause gain of function. We also identify suppressor missense mutations affecting basement membrane components type IV collagen (emb-9, let-2) and perlecan (unc-52), as well as a mutation affecting spectraplakin (vab-10), a component of the epidermal cytoskeleton. These suppressor alleles do not bypass the developmental requirement for core structural proteins of the basement membrane such as laminin or type IV collagen. In conclusion, putative gain of function alterations in matrix proteins or in cell-matrix receptors can overcome the requirement for certain basement membrane proteins in embryonic development, revealing previously unknown plasticity in the genetic requirements for the extracellular matrix.

     

    https://www.ncbi.nlm.nih.gov/pubmed/29440357

    category:実験 | by:shinjicomments(0) | -
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